The project is now fully implemented in Layar and I have tested it all out to confirm each marker is working correctly. There are 45 markers in my project (18 for Bovisand, 18 for Plymouth Route 1, 9 for Plymouth Route 2) and the videos play once the user is within 20m of them (except to the second half of the markers on the Bovisand route as the route goes back on itself, so to avoid confusion they don't auto play).
This evening I applied for Layar approval so hopefully it should be live to the public soon!
The video above shows me demoing it from my desk, and you can see the first marker from the medium route (amber) just up the road. Note my physical distance from the marker so I cannot view the video showing how to get to the next marker. So now here are some screen shots from within the app around the Basic (green) 2km route.